5/4/2023 0 Comments Sequencher mtdnaIndependent Nextera XT® (Illumina) NGS libraries were generated for each sample and sequenced in different MiSeq (Illumina) runs. A primer set that presents a perfect annealing with both mitochondrial and nuclear DNA was used to amplify the mitochondrial DNA region located between positions 67 and the corresponding region located inside a NUMT located in chromosome 1 (GRCh38.p12 Primary Assembly 631910-634079) that presents an identity of 98% with the corresponding region of mtDNA in two samples. Our purpose is to establish mitochondrial heteroplasmy detection limits, generating mixed bases at low frequencies by the PCR co-amplification of mtDNA and a nuclear insertion of mitochondrial origin (NUMT). ![]() Electronic address: the use of Next Generation Sequencing (NGS) in mitochondrial DNA (mtDNA) studies related to forensic and human genetics has contributed to the report of heteroplasmy at very low levels (lower than 1% and even 0.5%), their detection is not a straightforward process. 3 Unitat d'Antropologia Biològica, Departament de Biologia Animal, Biologia Vegetal i Ecologia, Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès, Barcelona, Spain GREAB - Research Group in Biological Anthropology, Spain.Electronic address: 1 Unitat d'Antropologia Biològica, Departament de Biologia Animal, Biologia Vegetal i Ecologia, Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès, Barcelona, Spain GREAB - Research Group in Biological Anthropology, Spain. 2 Unitat d'Antropologia Biològica, Departament de Biologia Animal, Biologia Vegetal i Ecologia, Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès, Barcelona, Spain GREAB - Research Group in Biological Anthropology, Spain Faculdade de Ciências e Tecnologia, Universidade dos Açores, Ponta Delgada, Portugal Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Portugal IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal.1 Unitat d'Antropologia Biològica, Departament de Biologia Animal, Biologia Vegetal i Ecologia, Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès, Barcelona, Spain GREAB - Research Group in Biological Anthropology, Spain.Sequencher from Gene Codes Corporation helps you go from DNA sequence data to results easier and FASTER than ever! Almost 20 years of daily use by biologists in labs around the world have refined Sequencher's tools and interface. ![]() Specialized tools for Forensic mtDNA profilingįor more information check out the Features page.Īre you frustrated that most bioinformatics software programs are hard to learn? Imagine DNA software that is so easy to learn and fast to use that you save hours a week, giving you more time in The Lab.What could you do with all that extra time? Powerful Reference Sequence and the Variance Table find SNPs quickly and easily.Įxtensive data import & export capabilities, including customizable GenBank Feature handling Multiple, configurable DNA assembly algorithmsįull support of sequence data confidence values Sequencher is the biologists choice for DNA sequence Assembly and analysis software.
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